CSIR - CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE
COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH
MINISTRY OF SCIENCE & TECHNOLOGY, GOVT. OF INDIA,
MYSURU - 570020
Molecular & Cell culture Techniques - PCR, Semi q- PCR, Cloning, Recombinant Protein expression and Purification, SDS � PAGE, Mammalian Cell culture, Virus culture, Bacterial culture, RFFIT, Cell based assays, maintenance of Cell lines, transfection, Confocal microscopy, Si RNA Gene Silencing, FACS analysis, adaptation of serum free media cells. BIO-PROCESS SKILLS Purification- By Affinity Chromatography- Ion exchange; Adsorption, Size exclusion Chromatography under GMP conditions Centrifugation - Handling and operation of High-Speed Cooling Centrifuges for separation of various bio-molecules, cell organelles (Mitochondria); Ultra-centrifuge for viral particles. Sterile Operation and activities-Operation and Handling of Laminar Air Flow Unit (LAFU) ; Bio-safety cabinets and other sterile activities under GMP conditions. ANALYTICAL SKILLS Biochemical & Immunological Techniques- FACS analysis, ELISA, HPLC, Routine biochemical tests, Endotoxin analysis; Biochemistry auto analyzers, Hormone analyzers, Hematology analyzers, Immuno precipitation, Western blot, RFFIT, Enzyme activity assays, adaptation of cells in serum free media, preparation of new adjuvants for vaccines, Vaccines development Genetics Collection of large data, SNP analysis, Patient interaction and case sheet analysis, Lymphocyte culture, Karyotyping, FISH technique. Animal Studies- Small animal handling like mice, rat and dissection of various organs. Computer SKILLS Computer Knowledge and Statistics Basic Bioinformatics Literature Collection, MS Office, working knowledge of SPSS statistical soft ware. Process Raw material and consumables consumption and tracking by intra-net software
Cardiovascular disorders, Metabolic disorders & Neuro degenerative diseases
B.Pulla reddy, B.Prasanna , Jaya Simha. Effects Of Fetal Bovine Serum Concentration On The Growth And Survival Of BHk 21 Cells. IJABPT,2015.,7,(2). Marada A, Allu PK, Murari A, PullaReddy B, Tammineni P, Thiriveedi VR, Danduprolu J, Sepuri NB. Mge1, a nucleotide exchange factor of Hsp70, acts as an oxidative sensor to regulate mitochondrial Hsp70 function. Mol Biol Cell 2013; 24(6) 692-703. http://www.ncbi.nlm.nih.gov/pubmed/23345595 Pulla Reddy BhoomiReddy, BMV. Srikanth babu, K.Venkata Karunakar, J. Yasovanthi, P. Sampath kumar, A. Sharth, A.Jyothy. Angiotensin II type 1 receptor gene polymorphism in myocardial infarction patients JRAAS 2009; 10, 3, 174-178.http://www.ncbi.nlm.nih.gov/pubmed/19713412 Pulla Reddy B, Srikanth Babu BM, Venkata Karunakar K, Yasovanthi J, Munshi A, Sampath Kumar P, Sharath A, Jyothy A. Angiotensin-converting enzyme gene variant and its levels: risk factors for myocardial infarction in a South Indian population. Singapore Med J. 2010; 51(7):576-81. http://www.ncbi.nlm.nih.gov/pubmed/20730398 B.M.V. Srikanth Babu, Reddy BP, V. Hari Sai Priya, Anjana Munshi, H. Surekha Rani, Suman Latha Gaddam, V. Dayasagar Rao, and A. Jyothy. Cytokine Gene Polymorphisms in the Susceptibility to Acute Coronary Syndrome. Genetic Testing and Molecular Biomarkers (GTMB) 2012 16(5) 359-365. http://www.ncbi.nlm.nih.gov/pubmed/22372709 Yasovanthi J, Venkata Karunakar K, Sri Manjari K, Pulla Reddy B, Ajeya Kumar P, Sesha Charyulu M, Aruna P, Narasimulu G, Jyothy A. Association of vitamin D receptor gene polymorphisms with BMD and their effect on 1, 25-dihydroxy vitamin D3 levels in pre- and postmenopausal South Indian women from Andhra Pradesh. Clin Chim Acta 2011., 412(7-8):541-4.http://www.ncbi.nlm.nih.gov/pubmed/21130756 Parthasarathireddy T, Pullareddy B, Sampath Kumar P warkanath K Murthy.Association between Apolipoprotein E Gene Polymorphism and Myocardial Infarction. Biochemical Genetics 2012; 51(526) 398-405. http://www.ncbi.nlm.nih.gov/pubmed/23371373 J.Yasovanthi, Bhoomi Reddy PR, K.Venkata Karunakar, Munshi Anjana, A.Venkateswari, G.Narasimulu and A.Jyothy. Association of estrogen receptor alpha gene polymorphisms with BMD and their affect on estradiol levels in pre and post menopausal women in south Indian population Andhra Pradesh from. Clinica Chimica Acta2010;411,597-600. http://www.ncbi.nlm.nih.gov/pubmed/20116372. Venkata Karunakar K, Madhavi G, Pulla Reddy B, Srikanth babu BMV, Yasovanthi J,Vijaya Lakshmi V, Jayanthi Ramesh, Jyothy A. Influence of tumor necrosis factor alpha, interferon gamma and interleukin 10 gene polymorphisms on diabetic peripheral neuropathy in South Indian patients with type 2 diabetes. �Cytokine, 2009; 47 (3), 173- 177. http://www.ncbi.nlm.nih.gov/pubmed/19608431 AnjanaMunshi, Shehnaz, SubhashKoul, Reddy BP, A. Jyothy. Angiotensin- converting enzyme insertion/ deletion polymorphism and the risk of ischemic stroke in Indian population. J of Neurological Sciences 2008; 272, 132� 135. http://www.ncbi.nlm.nih.gov/pubmed/18586276
